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Anti-GLT25D2 Polyclonal Antibody

Catalog No. TMAB-06525 Copy Product Info
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Anti-GLT25D2 Polyclonal Antibody is a Rabbit antibody targeting GLT25D2. Anti-GLT25D2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-GLT25D2 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-06525

Anti-GLT25D2 Polyclonal Antibody is a Rabbit antibody targeting GLT25D2. Anti-GLT25D2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-GLT25D2 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GLT25D2 Polyclonal Antibody is a Rabbit antibody targeting GLT25D2. Anti-GLT25D2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Chicken,Pig,Cow,Horse,Sheep)
Verified Activity
1. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GLT25D2 Polyclonal Antibody, Unconjugated (TMAB-06525) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLT25D2) Polyclonal Antibody, Unconjugated (TMAB-06525) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLT25D2) Polyclonal Antibody, Unconjugated (TMAB-06525) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum lumen.
Tissue SpecificityExpressed in brain and skeletal muscle.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundGLT25D2 is a 626 amino acid protein that localizes to endoplasmic reticulum lumen and belongs to the glycosyltransferase 25 family. Possessing beta-galactosyltransferase activity, GLT25D2 transports beta-galactose to hydroxylysine residues on collagen but lacks glucosyltransferase activity. GLT25D2 is expressed in skeletal muscle and brain, and is encoded by a gene located on human chromosome 1q25.3, a region associated with the hereditary prostate cancer (HPC1) locus. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GLT25D2
Antigen Species
Human
Gene Name
COLGALT2
Gene ID
Protein Name
Procollagen galactosyltransferase 2
Uniprot ID
Function
Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues on collagen.
Chemical Properties
Molecular WeightTheoretical: 70 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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