Anti-GLK Polyclonal Antibody is a Rabbit antibody targeting GLK. Anti-GLK Polyclonal Antibody can be used in WB,ICC/IF,FCM.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Horse,Rabbit)

1. Protein: rat liver lysates, 30 μg;
Primary: Anti-GLK (TMAB-06510) at 1: 200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000;
ECL excitated the fluorescence;
Predicted band size: 51 kD
Observed band size: 51 kD
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (GLK) polyclonal Antibody, Unconjugated (TMAB-06510) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
3. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-GLK antibody (TMAB-06510)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
4. Sample: Panc-1 (Human) Cell Lysate at 30 μg
Primary: Anti-GLK (TMAB-06510) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

WB: 1:500-2000; ICC/IF: 1:100-500; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GCK

Human

GCK

Hexokinase-4

Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.