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Anti-GLIS2 Polyclonal Antibody

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Catalog No. TMAB-06508 Copy Product Info
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Anti-GLIS2 Polyclonal Antibody is a Rabbit antibody targeting GLIS2. Anti-GLIS2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-GLIS2 Polyclonal Antibody

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2217-10 days7-10 days
100 µL$3717-10 days7-10 days
200 µL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GLIS2 Polyclonal Antibody is a Rabbit antibody targeting GLIS2. Anti-GLIS2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLIS2) Polyclonal Antibody, Unconjugated (TMAB-06508) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLIS2) Polyclonal Antibody, Unconjugated (TMAB-06508) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus speckle. Cytoplasm.
Tissue SpecificityExpressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundGLIS2 is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family. By recruiting the corepressors CtBP1 and HDAC3, GLIS2 represses the transcriptional activation mediated by ∫-catenin in the Wnt pathway. GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation. Mutations of GLIS2 may be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis. GLIS2 contains five tandem Cys(2)-His(2) zinc finger motifs that exhibit the highest homology to those of members of the GLI and Zic subfamilies of Krüppel-like proteins. GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GLIS2
Antigen Species
Human
Gene Name
GLIS2
Gene ID
Protein Name
Zinc finger protein GLIS2
Uniprot ID
Function
Can act either as a transcription repressor or as a transcription activator, depending on the cell context. Represses the transcriptional activation mediated by CTNNB1 in the Wnt pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.
Chemical Properties
Molecular WeightTheoretical: 56 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-GLIS2 Polyclonal Antibody molecular weight