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Anti-GLI4 Polyclonal Antibody

Catalog No. TMAB-06505 Copy Product Info
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Anti-GLI4 Polyclonal Antibody is a Rabbit antibody targeting GLI4. Anti-GLI4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GLI4 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-06505

Anti-GLI4 Polyclonal Antibody is a Rabbit antibody targeting GLI4. Anti-GLI4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GLI4 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GLI4 Polyclonal Antibody is a Rabbit antibody targeting GLI4. Anti-GLI4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Pig,Cow,Horse)
Verified Activity
1. Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GLI4 Polyclonal Antibody, Unconjugated (TMAB-06505) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: A549 Cell (Human) Lysate at 30 μg
Primary: Anti-GLI4 (TMAB-06505) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundZinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-4, also known as HKR4, is a 376 amino acid protein that localizes to the nucleus and contains seven C2H2-type zinc fingers. Belonging to the krueppel C2H2-type zinc-finger protein family, GLI-4 may function as a transcriptional regulator, effectively activating or repressing the transcription of target genes. The gene encoding GLI-4 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GLI4
Antigen Species
Human
Gene Name
GLI4
Gene ID
2738
Protein Name
Zinc finger protein GLI4
Uniprot ID
P10075
Chemical Properties
Molecular WeightTheoretical: 41 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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