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Anti-GHRHR Polyclonal Antibody 2
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Catalog No. TMAB-06470
Anti-GHRHR Polyclonal Antibody 2 is a Rabbit antibody targeting GHRHR. Anti-GHRHR Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GHRHR Polyclonal Antibody 2
Anti-GHRHR Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-06470
Anti-GHRHR Polyclonal Antibody 2 is a Rabbit antibody targeting GHRHR. Anti-GHRHR Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
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Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-GHRHR Polyclonal Antibody 2 is a Rabbit antibody targeting GHRHR. Anti-GHRHR Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Adrenal gland (rat) Lysate at 40 μg panc-1 (Human) Cell Lysate at 40 μg Primary: Anti-GHRHR(TMAB-06470) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-GHRHR Polyclonal Antibody, Unconjugated (TMAB-06470) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. |
| Tissue Specificity | Pituitary gland. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | GHRH-R is a seven transmembrane domain protein that localizes to the somatotroph of the pituitary. GHRH-R plays an important role in growth and acts as a high-affinity receptor for GHRH. Binding of GHRH leads to the coupling of GHRH-R to G-protein which stimulates increased adenylyl cyclase activity and the accumulation of cAMP leading to the synthesis and release of growth hormone and somatotroph proliferation. In addition, this signalling pathway may have direct action in fetal/placental development, reproduction and immune function. GHRH and GHRH-R may also play a role in the regulation of non-rapid eye movement sleep (NREMS). The expression of GHRH-R is dependent on the presence of the POU domain factor Pit-1. Mutations in the gene encoding this protein can result in isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, and anterior pituitary hypoplasia (APH). |
| Immunogen | KLH conjugated synthetic peptide: Rabbit GHRHR |
| Antigen Species | Rabbit |
| Gene Name | GHRHR |
| Gene ID | |
| Protein Name | Growth hormone-releasing hormone receptor |
| Function | Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. |
| Molecular Weight | Theoretical: 45 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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