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Anti-GFP Tag Monoclonal Antibody (7N190)
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Catalog No. TMAT-1024 Copy Product Info
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Anti-GFP Tag Monoclonal Antibody (7N190) is a Mouse antibody targeting GFP Tag. Anti-GFP Tag Monoclonal Antibody (7N190) can be used in IHC,FCM.
Anti-GFP Tag Monoclonal Antibody
(7N190)
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| Description | Anti-GFP Tag Monoclonal Antibody (7N190) is a Mouse antibody targeting GFP Tag. Anti-GFP Tag Monoclonal Antibody (7N190) can be used in IHC,FCM. |
| Ig Type | IgG2a |
| Clone | 7N190 |
| Reactivity | Tag |
| Application | |
| Recommended Dose | IHC: 1/200-1/1000; FCM: 1/200-1/400 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Purified by protein A or protein G |
| Appearance | Liquid |
| Formulation | PBS with 0.05% sodium azide |
| Concentration | 1 mg/mL |
| Research Background | GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. |
| Conjucates | Unconjugated |
| Immunogen | Purified recombinant fragment of GFP expressed in E. Coli. |
| Antigen Species | Aequorea victoria |
| Gene Name | GFP |
| Protein Name | Green fluorescent protein |
| Uniprot ID |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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