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Anti-GFAP Antibody (3A643)
😃Good
Catalog No. TMAB-00763
Alias Glial Fibrillary Acidic Protein, GFAP
Anti-GFAP Antibody (3A643) is a Rabbit antibody targeting GFAP. Anti-GFAP Antibody (3A643) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GFAP Antibody (3A643)
Anti-GFAP Antibody (3A643)
😃Good
Catalog No. TMAB-00763Alias Glial Fibrillary Acidic Protein, GFAP
Anti-GFAP Antibody (3A643) is a Rabbit antibody targeting GFAP. Anti-GFAP Antibody (3A643) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $153 | 7-10 days | |
| 50 μL | $273 | 7-10 days | |
| 100 μL | $487 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-GFAP Antibody (3A643) is a Rabbit antibody targeting GFAP. Anti-GFAP Antibody (3A643) can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Glial Fibrillary Acidic Protein, GFAP |
| Ig Type | IgG |
| Clone | 3A643 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. 25 μg total protein per Lane of various lysates probed with GFAP monoclonal antibody, unconjugated (TMAB-00763) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1:20000; IHC-P: 1:100-1:500; IHC-Fr: 1:100-1:500; IF: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Note=Associated with intermediate filaments. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Immunogen | A synthesized peptide: human GFAP |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Intermediate Filaments,Astrocyte marker,GFAP,Astrocyte,Glial |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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