Anti-GFAP Antibody (3A643) is a Rabbit antibody targeting GFAP. Anti-GFAP Antibody (3A643) can be used in IF,IHC-Fr,IHC-P,WB.

IgG

3A643

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. 25 μg total protein per Lane of various lysates probed with GFAP monoclonal antibody, unconjugated (TMAB-00763) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-1:20000; IHC-P: 1:100-1:500; IHC-Fr: 1:100-1:500; IF: 1:100-1:500

Monoclonal

A synthesized peptide: human GFAP

Human

GFAP

Glial fibrillary acidic protein

Intermediate Filaments,Astrocyte marker,GFAP,Astrocyte,Glial

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.