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Anti-Gelsolin Polyclonal Antibody

Catalog No. TMAB-06423 Copy Product Info
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Anti-Gelsolin Polyclonal Antibody is a Rabbit antibody targeting Gelsolin. Anti-Gelsolin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Gelsolin Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-06423

Anti-Gelsolin Polyclonal Antibody is a Rabbit antibody targeting Gelsolin. Anti-Gelsolin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Gelsolin Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Gelsolin Polyclonal Antibody is a Rabbit antibody targeting Gelsolin. Anti-Gelsolin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gelsolin) Polyclonal Antibody, Unconjugated (TMAB-06423) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gelsolin) Polyclonal Antibody, Unconjugated (TMAB-06423) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform 2: Cytoplasm, cytoskeleton. Isoform 1: Secreted.
Tissue SpecificityPhagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research Backgroundbs-1160P is one synthetic peptide derived from human Gelsolin. Gelsolin is a calcium dependent actin binding protein. It is a potent modulator of actin filament length and gelation. Gelsolin has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called actin depolymerizing factor (ADF) or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25 amino acid residue extension at the NH2 terminus in the human plasma gelsolin, which appears to account for the difference in relative molecular weights.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Gelsolin
Antigen Species
Human
Gene Name
GSN
Gene ID
Protein Name
Gelsolin
Uniprot ID
Function
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Chemical Properties
Molecular WeightTheoretical: 80 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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