Anti-Gelsolin Antibody (3V993) is a Rabbit antibody targeting Gelsolin. Anti-Gelsolin Antibody (3V993) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

3V993

Human,Mouse

1. Sample:
Lane 1: Plasma (Mouse) at 20 μg
Lane 2: HepG2 (Human) Cell Lysate at 30 μg
Lane 3: Huvec (Human) Cell Lysate at 30 μg
Lane 4: Hela (Human) Cell Lysate at 30 μg
Lane 5: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-Gelsolin (TMAB-06424) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90 kD
2. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: Huvec (Human) Cell Lysate at 30 μg
Lane 3: Hela (Human) Cell Lysate at 30 μg
Lane 4: U251 (Human) Cell Lysate at 30 μg
Lane 5: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 6: Siha (Human) Cell Lysate at 30 μg
Primary: Anti-Gelsolin (TMAB-06424) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90 kD
3. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gelsolin) Monoclonal Antibody, Unconjugated (TMAB-06424) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200

Monoclonal

Recombinant Protein: human Gelsolin protein

Human

GSN

Gelsolin

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.