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Anti-GDNF Polyclonal Antibody
😃Good
Catalog No. TMAB-00760
Anti-GDNF Polyclonal Antibody is a Rabbit antibody targeting GDNF. Anti-GDNF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GDNF Polyclonal Antibody
Anti-GDNF Polyclonal Antibody
😃Good
Catalog No. TMAB-00760
Anti-GDNF Polyclonal Antibody is a Rabbit antibody targeting GDNF. Anti-GDNF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-GDNF Polyclonal Antibody is a Rabbit antibody targeting GDNF. Anti-GDNF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GDNF Polyclonal Antibody, Unconjugated (TMAB-00760) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (TMAB-00760) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (TMAB-00760) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Spinal cord (Mouse) Lysate at 40 μg Lane 3: Cerebrum (Rat) Lysate at 40 μg Lane 4: Spinal cord (Rat) Lysate at 40 μg Lane 5: U87MG (Human) Cell Lysate at 30 μg Lane 6: U251 (Human) Cell Lysate at 30 μg Primary: Anti-GDNF (TMAB-00760) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25/20 kDa Observed band size: 28 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Neurobiology. Neurotrophins. Neuroscience. This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. |
| Immunogen | KLH conjugated synthetic peptide: human GDNF |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Kidney development,Neurotrophins,Amyotrophic lateral sclerosis,Parkinson's disease |
| Molecular Weight | Theoretical: 15 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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