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Anti-GDF-3 Antibody (5D320) is a Rabbit antibody targeting GDF-3. Anti-GDF-3 Antibody (5D320) can be used in IHC-P,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $92 | 7-10 days | |
100 μL | $270 | 7-10 days |
Description | Anti-GDF-3 Antibody (5D320) is a Rabbit antibody targeting GDF-3. Anti-GDF-3 Antibody (5D320) can be used in IHC-P,ICC/IF. |
Alias | UNQ222/PRO248, MCOPCB6, MCOP7, KFS3 |
Ig Type | Monoclonal Rabbit IgG |
Clone | 5D320 |
Reactivity | Human |
Verified Activity | 1. Immunochemical staining of human GDF3 in human testis with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human GDF3 in human breast carcinoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). 3. Immunofluorescence staining of GDF3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GDF3 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
Application | IHC-P,ICC/IF |
Recommended Dose | IHC-P: 1:50-1:200; ICC-IF: 1:20-1:100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human GDF-3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The GDF3 gene plays a fundamental role in embryonic morphogenesis. GDF3 expression is significantly decreased in human breast cancer tissues, and reconstitution of GDF3 in breast cancer may be a potential therapeutic approach to inhibit aggressive growth of breast cancer. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide corresponding to the center region of the Human GDF-3 |
Antigen Species | Human |
Biology Area | Early Mesodermal Lineage Markers |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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