Anti-GCSAM Antibody (3D490) is a Rabbit antibody targeting GCSAM. Anti-GCSAM Antibody (3D490) can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM,WB.

IgG

3D490

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with GCSAM monoclonal antibody, unconjugated (TMAB-06399) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Raji (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD163L1 antibody (TMAB-06399, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCSAM Monoclonal Antibody, Unconjugated (TMAB-06399) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCSAM Monoclonal Antibody, Unconjugated (TMAB-06399) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCSAM Monoclonal Antibody, Unconjugated (TMAB-06399) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
6. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (GCSAM) monoclonal Antibody, unconjugated (TMAB-06399) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1:50-100; WB: 1:500-2000

Monoclonal

A synthesized peptide: human GCSAM

Human

GCSAM

Germinal center-associated signaling and motility protein

Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK.