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Anti-GCNT1 Polyclonal Antibody
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Catalog No. TMAB-06393
Anti-GCNT1 Polyclonal Antibody is a Rabbit antibody targeting GCNT1. Anti-GCNT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GCNT1 Polyclonal Antibody
Anti-GCNT1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-06393
Anti-GCNT1 Polyclonal Antibody is a Rabbit antibody targeting GCNT1. Anti-GCNT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-GCNT1 Polyclonal Antibody is a Rabbit antibody targeting GCNT1. Anti-GCNT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GCNT1) Polyclonal Antibody, Unconjugated (TMAB-06393) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Rat Lymph node tissue lysates Lane 2: Human SW480 cell lysates Lane 3: Human TT cell lysates Lane 4: Human U87MG cell lysates Lane 5: Human MCF-7 cell lysates Primary: Anti-GCNT1 (TMAB-06393) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD 3. Sample: U87MG (Human) Cell Lysate at 30 μg Primary: Anti-GCNT1 (TMAB-06393) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 52 kD 4. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-GCNT1 (TMAB-06393) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Golgi apparatus membrane. |
| Tissue Specificity | Highly expressed in activated T-lymphocytes and myeloid cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | GCNT1 is expressed in a variety of cell types, including lymphocytes and mucin-producing cells. Specifically, GCNT1 expression in leukocytes regulates the synthesis of core 2 O-glycans that carry sialyl-Lewis x (sLex) oligosaccharides, which confer high affinity binding to Selectins. Downregulation of Selectin ligand expression levels has been shown to inhibit tissue infiltration. Therefore, GCNT1 represents a potential drug target for the treatment of inflammatory disorders and other pathologies involving Selectins. |
| Immunogen | KLH conjugated synthetic peptide: Human GCNT1 |
| Antigen Species | Human |
| Gene Name | GCNT1 |
| Gene ID | |
| Protein Name | beta-1, 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase |
| Uniprot ID | |
| Function | Forms critical branches in O-glycans. |
| Molecular Weight | Theoretical: 50 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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