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Anti-GCNF Antibody (6U757) (Synonyms: RTR, Retinoid receptor-related testis-specific receptor, Retinoid recept...)

Catalog No. TMAC-01638 Copy Product Info
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Anti-GCNF Antibody (6U757) is a Mouse antibody targeting GCNF. Anti-GCNF Antibody (6U757) can be used in FCM,ICC,IHC,WB.

Anti-GCNF Antibody (6U757)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01638
Synonyms RTR, Retinoid receptor-related testis-specific receptor, Retinoid receptor related testis specific receptor, Retinoic acid receptor related testis associated receptor, Orphan nuclear receptor NR6A1, Nuclear receptor subfamily 6 group A member 1, NR6A1_HUMAN, nr6a1, NR61, NR 61, hRTR, hGCNF, Germ cell nuclear factor, GCNF1, GCNF 1, GCNF

Anti-GCNF Antibody (6U757) is a Mouse antibody targeting GCNF. Anti-GCNF Antibody (6U757) can be used in FCM,ICC,IHC,WB.

Anti-GCNF Antibody
(6U757)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GCNF Antibody (6U757) is a Mouse antibody targeting GCNF. Anti-GCNF Antibody (6U757) can be used in FCM,ICC,IHC,WB.
SynonymsRTR, Retinoid receptor-related testis-specific receptor, Retinoid receptor related testis specific receptor, Retinoic acid receptor related testis associated receptor, Orphan nuclear receptor NR6A1, Nuclear receptor subfamily 6 group A member 1, NR6A1_HUMAN, nr6a1, NR61, NR 61, hRTR, hGCNF, Germ cell nuclear factor, GCNF1, GCNF 1, GCNF
Clone
6U757
Reactivity
Human
Verified Activity
1. Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1,000 dilution.
2. Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1,000 dilution.
3. Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1,000 dilution. Positive control:Lane1: K562, Lane2: NTERA-2, Lane3: HEK293, Lane4: HUVE-12, Lane5: HeLa.
4. Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.
6. ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-1000; IHC: 1:100-500; ICC: 1:50-200; FCM: 1:100-200
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
Research BackgroundGerm cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 54 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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