Anti-Gclc Polyclonal Antibody is a Rabbit antibody targeting Gclc. Anti-Gclc Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (TMAB-00754) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Kidney (Mouse) Lysate at 40 μg
Kidney (Rat) Lysate at 40 μg
Primary: Anti-GCLC (TMAB-00754) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Human HepG2 cell lysates
Primary: Anti-GCLC (TMAB-00754) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human GCLC

Human

GCLC

Glutamate--cysteine ligase catalytic subunit

Mitochondrial,Oxidative stress,Mitochondrial markers,Oxidative Stress,Other Antibodies