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Anti-Gclc Polyclonal Antibody
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Catalog No. TMAB-00754
Alias Glutamate--cysteine ligase catalytic subunit, Glclc, GCS heavy chain, Gclc, Gamma-glutamylcysteine synthetase, Gamma-ECS
Anti-Gclc Polyclonal Antibody is a Rabbit antibody targeting Gclc. Anti-Gclc Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-Gclc Polyclonal Antibody
Anti-Gclc Polyclonal Antibody
😃Good
Catalog No. TMAB-00754Alias Glutamate--cysteine ligase catalytic subunit, Glclc, GCS heavy chain, Gclc, Gamma-glutamylcysteine synthetase, Gamma-ECS
Anti-Gclc Polyclonal Antibody is a Rabbit antibody targeting Gclc. Anti-Gclc Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Gclc Polyclonal Antibody is a Rabbit antibody targeting Gclc. Anti-Gclc Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Glutamate--cysteine ligase catalytic subunit, Glclc, GCS heavy chain, Gclc, Gamma-glutamylcysteine synthetase, Gamma-ECS |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (TMAB-00754) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Kidney (Mouse) Lysate at 40 μg Kidney (Rat) Lysate at 40 μg Primary: Anti-GCLC (TMAB-00754) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa 3. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Rat Liver tissue lysates Lane 3: Human HepG2 cell lysates Primary: Anti-GCLC (TMAB-00754) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. Defects in GCLC are the cause of hemolytic anemia. |
| Immunogen | KLH conjugated synthetic peptide: human GCLC |
| Antigen Species | Human |
| Gene Name | GCLC |
| Gene ID | |
| Protein Name | Glutamate--cysteine ligase catalytic subunit |
| Uniprot ID | |
| Biology Area | Mitochondrial,Oxidative stress,Mitochondrial markers,Oxidative Stress,Other Antibodies |
| Molecular Weight | Theoretical: 73 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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