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Anti-Gclc Polyclonal Antibody

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Catalog No. TMAB-00754
Alias Glutamate--cysteine ligase catalytic subunit, Glclc, GCS heavy chain, Gclc, Gamma-glutamylcysteine synthetase, Gamma-ECS

Anti-Gclc Polyclonal Antibody is a Rabbit antibody targeting Gclc. Anti-Gclc Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Gclc Polyclonal Antibody

Anti-Gclc Polyclonal Antibody

😃Good
Catalog No. TMAB-00754Alias Glutamate--cysteine ligase catalytic subunit, Glclc, GCS heavy chain, Gclc, Gamma-glutamylcysteine synthetase, Gamma-ECS
Anti-Gclc Polyclonal Antibody is a Rabbit antibody targeting Gclc. Anti-Gclc Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-Gclc Polyclonal Antibody is a Rabbit antibody targeting Gclc. Anti-Gclc Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasGlutamate--cysteine ligase catalytic subunit, Glclc, GCS heavy chain, Gclc, Gamma-glutamylcysteine synthetase, Gamma-ECS
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (TMAB-00754) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Kidney (Mouse) Lysate at 40 μg
Kidney (Rat) Lysate at 40 μg
Primary: Anti-GCLC (TMAB-00754) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Human HepG2 cell lysates
Primary: Anti-GCLC (TMAB-00754) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GCLC
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Mitochondrial,Oxidative stress,Mitochondrial markers,Oxidative Stress,Other Antibodies
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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