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Anti-GBA3 Antibody (9P236)
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Catalog No. TMAY-01922
Alias MGC126878, MGC104276, KLrP, glucosidase, β, acid 3 (gene/pseudogene), glucosidase, beta, acid 3 (gene/pseudogene), GLUC, GBA3, CBGL1, CBG
Anti-GBA3 Antibody (9P236) is a Rabbit antibody targeting GBA3. Anti-GBA3 Antibody (9P236) can be used in FCM,ICC/IF.
Anti-GBA3 Antibody (9P236)
Anti-GBA3 Antibody (9P236)
😃Good
Catalog No. TMAY-01922Alias MGC126878, MGC104276, KLrP, glucosidase, β, acid 3 (gene/pseudogene), glucosidase, beta, acid 3 (gene/pseudogene), GLUC, GBA3, CBGL1, CBG
Anti-GBA3 Antibody (9P236) is a Rabbit antibody targeting GBA3. Anti-GBA3 Antibody (9P236) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | MGC126878, MGC104276, KLrP, glucosidase, β, acid 3 (gene/pseudogene), glucosidase, beta, acid 3 (gene/pseudogene), GLUC, GBA3, CBGL1, CBG |
| Ig Type | Rabbit IgG |
| Clone | 9P236 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of Human GBA3 expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human GBA3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Confocal immunofluorescence analysis of Human GBA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human GBA3 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human GBA3 (rh GBA3; TMPY-01579; NP_066024.1; Met1-Leu469). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide. |
| Conjucates | Unconjugated |
| Others Formats | FITC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Protein: Human GBA3 protein (TMPY-01579) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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