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Anti-GBA3 Antibody (9P236)

😃Good
Catalog No. TMAY-01922
Alias MGC126878, MGC104276, KLrP, glucosidase, β, acid 3 (gene/pseudogene), glucosidase, beta, acid 3 (gene/pseudogene), GLUC, GBA3, CBGL1, CBG

Anti-GBA3 Antibody (9P236) is a Rabbit antibody targeting GBA3. Anti-GBA3 Antibody (9P236) can be used in FCM,ICC/IF.

Anti-GBA3 Antibody (9P236)

Anti-GBA3 Antibody (9P236)

😃Good
Catalog No. TMAY-01922Alias MGC126878, MGC104276, KLrP, glucosidase, β, acid 3 (gene/pseudogene), glucosidase, beta, acid 3 (gene/pseudogene), GLUC, GBA3, CBGL1, CBG
Anti-GBA3 Antibody (9P236) is a Rabbit antibody targeting GBA3. Anti-GBA3 Antibody (9P236) can be used in FCM,ICC/IF.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Anti-GBA3 Antibody (9P236) is a Rabbit antibody targeting GBA3. Anti-GBA3 Antibody (9P236) can be used in FCM,ICC/IF.
AliasMGC126878, MGC104276, KLrP, glucosidase, β, acid 3 (gene/pseudogene), glucosidase, beta, acid 3 (gene/pseudogene), GLUC, GBA3, CBGL1, CBG
Ig Type
Monoclonal Rabbit IgG
Clone
9P236
Reactivity
Human
Verified Activity
1. Flow cytometric analysis of Human GBA3 expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human GBA3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

2. Confocal immunofluorescence analysis of Human GBA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human GBA3 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human GBA3 (rh GBA3; TMPY-01579; NP_066024.1; Met1-Leu469).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC/PE
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant Human GBA3 protein (TMPY-01579)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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