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Anti-GATA4 Antibody (5B64) is a Rabbit antibody targeting GATA4. Anti-GATA4 Antibody (5B64) can be used in WB,ICC/IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
| Description | Anti-GATA4 Antibody (5B64) is a Rabbit antibody targeting GATA4. Anti-GATA4 Antibody (5B64) can be used in WB,ICC/IF,FCM. |
| Ig Type | IgG |
| Clone | 5B64 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (GATA4) monoclonal Antibody, unconjugated (TMAB-06359) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. 25 μg total protein per lane of various lysates (see on figure) probed with GATA4 monoclonal antibody, unconjugated (TMAB-06359) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function.In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. |
| Immunogen | A synthesized peptide: human GATA4 |
| Antigen Species | Human |
| Gene Name | GATA4 |
| Gene ID | |
| Protein Name | Transcription factor GATA-4 |
| Uniprot ID | |
| Function | Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement. |
| Molecular Weight | Theoretical: 45 kDa. Actual: 54 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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