Shopping Cart
Remove All
Your shopping cart is currently empty
Anti-GATA3 Antibody (3N332)
Copy Product Info
😃Good
Catalog No. TMAB-06355
Anti-GATA3 Antibody (3N332) is a Rabbit antibody targeting GATA3. Anti-GATA3 Antibody (3N332) can be used in IF, IHC-Fr, IHC-P, WB.
Anti-GATA3 Antibody (3N332)
Anti-GATA3 Antibody (3N332)
Copy Product Info😃Good
Catalog No. TMAB-06355
Anti-GATA3 Antibody (3N332) is a Rabbit antibody targeting GATA3. Anti-GATA3 Antibody (3N332) can be used in IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
Add to Cart
Add to Quotation
In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-GATA3 Antibody (3N332) is a Rabbit antibody targeting GATA3. Anti-GATA3 Antibody (3N332) can be used in IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| Clone | 3N332 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GATA3) Monoclonal Antibody, Unconjugated (TMAB-06355) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. 25 μg total protein per lane of various lysates (see on figure) probed with GATA3 monoclonal antibody, unconjugated (TMAB-06355) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GATA3 Monoclonal Antibody, Unconjugated (TMAB-06355) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GATA3 Monoclonal Antibody, Unconjugated (TMAB-06355) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GATA3 Monoclonal Antibody, Unconjugated (TMAB-06355) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rouse Red), DAPI (blue) was used to stain the cell nuclei. 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GATA3 Monoclonal Antibody, Unconjugated (TMAB-06355) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rouse Red), DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | T-cells and endothelial cells. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | PBS, Glycerol, BSA. |
| Concentration | 1mg/ml |
| Research Background | Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites. |
| Immunogen | A synthesized peptide: human GATA3 |
| Gene Name | GATA3 |
| Gene ID | |
| Protein Name | Trans-acting T-cell-specific transcription factor GATA-3 |
| Function | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. |
| Molecular Weight | Theoretical: 49 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
Preview
Related Tags: buy Anti-GATA3 Antibody (3N332) | purchase Anti-GATA3 Antibody (3N332) | Anti-GATA3 Antibody (3N332) cost | order Anti-GATA3 Antibody (3N332) | Anti-GATA3 Antibody (3N332) molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.