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Anti-GAPDH Antibody (6G48)
😃Good
Catalog No. TMAY-00247
Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (6G48) is a Rabbit antibody targeting GAPDH. Anti-GAPDH Antibody (6G48) can be used in WB,IP.
Anti-GAPDH Antibody (6G48)
Anti-GAPDH Antibody (6G48)
😃Good
Catalog No. TMAY-00247Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (6G48) is a Rabbit antibody targeting GAPDH. Anti-GAPDH Antibody (6G48) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Alias | HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD |
| Ig Type | Rabbit IgG |
| Clone | 6G48 |
| Reactivity | Human |
| Verified Activity | 1. Anti-GAPDH rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: HepG2 Whole Cell Lysate. -Lane D: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:36 kDa. -Observed band size:36 kDa. 2. GAPDH was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate. -Lane C:0.5 mg Jurkat Whole Cell Lysate. -4 µL anti-GAPDH rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-GAPDH rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size:36 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: IP:1-5 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human GAPDH (rh GAPDH; TMPY-02446; P04406; Met1-Glu335). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human GAPDH Protein (TMPY-02446) |
| Antigen Species | Human |
| Biology Area | Adaptor Proteins |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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