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Anti-GAPDH Antibody (1V316) (Alias: HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD)

Catalog No. TMAC-01615 Copy Product Info
🥰Excellent
Anti-GAPDH Antibody (1V316) is a Mouse antibody targeting GAPDH. Anti-GAPDH Antibody (1V316) can be used in ICC,WB.

Anti-GAPDH Antibody (1V316)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01615
Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD

Anti-GAPDH Antibody (1V316) is a Mouse antibody targeting GAPDH. Anti-GAPDH Antibody (1V316) can be used in ICC,WB.

Anti-GAPDH Antibody
(1V316)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4267-10 days7-10 days
In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GAPDH Antibody (1V316) is a Mouse antibody targeting GAPDH. Anti-GAPDH Antibody (1V316) can be used in ICC,WB.
SynonymsHEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Clone
1V316
Reactivity
Human,Mouse,Rat,zebrafish,rabbit
Verified Activity
1. Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution. Positive control: Lane 1: HepG2, Lane 2: Hela, Lane 3: PC12, Lane 4: NIH/3T3, Lane 5: MCF-7, Lane 6: Rabbit liver, Lane 7: Zebrafish.
2. ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:5000-10000; ICC: 1:200
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProL affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Peptide
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 36 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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