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Anti-ganglioside GM1 Polyclonal Antibody

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Catalog No. TMAB-00738

Anti-ganglioside GM1 Polyclonal Antibody is a Rabbit antibody targeting ganglioside GM1. Anti-ganglioside GM1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-ganglioside GM1 Polyclonal Antibody

Anti-ganglioside GM1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00738
Anti-ganglioside GM1 Polyclonal Antibody is a Rabbit antibody targeting ganglioside GM1. Anti-ganglioside GM1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$374 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF,FCM

Reactivity: Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)
Verified Activity
1. Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-ganglioside GM1/AF647 Conjugated antibody (TMAB-00738-AF647), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange),used under the same conditions.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GM1 (GS) Polyclonal Antibody, Unconjugated (TMAB-00738) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationPlasma membrane
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundA member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
Antigen Details
Immunogen
KLH conjugated ganglioside GM1 purified from pig
Antigen Species
Pig
Biology Area
Other Antibodies,Neurodegenerative disease
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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