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Anti-ganglioside GM1 Polyclonal Antibody is a Rabbit antibody targeting ganglioside GM1. Anti-ganglioside GM1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF,FCM Reactivity: Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,GuineaPig) |
Ig Type | IgG |
Reactivity | Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,GuineaPig) |
Verified Activity | 1. Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-ganglioside GM1/AF647 Conjugated antibody (TMAB-00738-AF647), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange),used under the same conditions. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GM1 (GS) Polyclonal Antibody, Unconjugated (TMAB-00738) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Plasma membrane |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function. |
Immunogen | KLH conjugated ganglioside GM1 purified from pig |
Antigen Species | Pig |
Biology Area | Other Antibodies,Neurodegenerative disease |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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