Anti-gamma Sarcoglycan Antibody (3D462) is a Rabbit antibody targeting gamma Sarcoglycan. Anti-gamma Sarcoglycan Antibody (3D462) can be used in IF, IHC-Fr, IHC-P, IP, WB.

IgG

3D462

Human

1. Western blot analysis of Human skeletal muscle lysate. Using gamma Sarcoglycan (TMAB-06322) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with gamma Sarcoglycan Monoclonal Antibody, Unconjugated (TMAB-06322) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with gamma Sarcoglycan Monoclonal Antibody, Unconjugated (TMAB-06322) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with gamma Sarcoglycan Monoclonal Antibody, Unconjugated (TMAB-06322) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:1000-2000

Monoclonal

A synthesized peptide: human gamma Sarcoglycan

Human

SGCG

gamma-sarcoglycan

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.