Anti-Gamma-Adaptin Polyclonal Antibody is a Rabbit antibody targeting Gamma-Adaptin. Anti-Gamma-Adaptin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit)

1. Tissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin, Unconjugated (TMAB-06326) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (TMAB-06326) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (TMAB-06326) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Cell culture (MCF7); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (TMAB-06326) at 1: 200 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes and DAPI staining.
5. Cell culture (SW480); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (TMAB-06326) at 1: 200 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes and DAPI staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (TMAB-06326) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Sample:
293T (Human) Cell Lysate at 30 μg
JurkaT (Human) Cell Lysate at 30 μg
Primary: Anti-Gamma-Adaptin (TMAB-06326) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Gamma-Adaptin

Human

AP1G1

AP-1 complex subunit gamma-1

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.