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Anti-GALT Polyclonal Antibody is a Rabbit antibody targeting GALT. Anti-GALT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-GALT Polyclonal Antibody is a Rabbit antibody targeting GALT. Anti-GALT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Cow) |
| Verified Activity | 1. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-GALT (TMAB-06315) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD 2. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GALT) Polyclonal Antibody, Unconjugated (TMAB-06315) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | GALT (Galactose 1 phosphate uridyl transferase) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP glucose + galactose 1 phosphate to glucose 1 phosphate + UDP galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. |
| Immunogen | KLH conjugated synthetic peptide: human GALT |
| Antigen Species | Human |
| Gene Name | GALT |
| Gene ID | |
| Protein Name | Galactose-1-phosphate uridylyltransferase |
| Uniprot ID |
| Molecular Weight | Theoretical: 43 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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