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Anti-GALT Polyclonal Antibody

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Catalog No. TMAB-06315

Anti-GALT Polyclonal Antibody is a Rabbit antibody targeting GALT. Anti-GALT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GALT Polyclonal Antibody

Anti-GALT Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-06315
Anti-GALT Polyclonal Antibody is a Rabbit antibody targeting GALT. Anti-GALT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-GALT Polyclonal Antibody is a Rabbit antibody targeting GALT. Anti-GALT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Cow)
Verified Activity
1. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-GALT (TMAB-06315) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GALT) Polyclonal Antibody, Unconjugated (TMAB-06315) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundGALT (Galactose 1 phosphate uridyl transferase) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP glucose + galactose 1 phosphate to glucose 1 phosphate + UDP galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GALT
Antigen Species
Human
Gene Name
GALT
Gene ID
Protein Name
Galactose-1-phosphate uridylyltransferase
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 43 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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