Anti-Galectin 8 Antibody (3L848) is a Rabbit antibody targeting Galectin 8. Anti-Galectin 8 Antibody (3L848) can be used in FCM, ICC/IF.

IgG

3L848

Human

1. The PANC-1 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Galectin 8 antibody (TMAB-06299, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed PANC-1 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Galectin 8) monoclonal Antibody, unconjugated (TMAB-06299) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1:50-100; ICC/IF=1:50-200

Monoclonal

KLH conjugated synthetic peptide: human Galectin 8

Human

LGALS8

Galectin-8