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Anti-Galectin-3/LGALS3 Antibody (5Z505)
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Catalog No. TMAY-00560
Anti-Galectin-3/LGALS3 Antibody (5Z505) is a Rabbit antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Antibody (5Z505) can be used in WB,IP.
Anti-Galectin-3/LGALS3 Antibody (5Z505)
Anti-Galectin-3/LGALS3 Antibody (5Z505)
😃Good
Catalog No. TMAY-00560
Anti-Galectin-3/LGALS3 Antibody (5Z505) is a Rabbit antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Antibody (5Z505) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Ig Type | Rabbit IgG |
| Clone | 5Z505 |
| Reactivity | Human |
| Verified Activity | 1. Anti-Galectin3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: SW480 Whole Cell Lysate. -Lane C: A431 Whole Cell Lysate. -Lane D: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:30 kDa. 2. Galectin3 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg MCF-7 Whole Cell Lysate. -Lane C:0.5 mg A431 Whole Cell Lysate. -2 µL anti-Galectin3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Galectin3 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 30 kDa. -Observed band size: 30 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Galectin-3 / LGALS3 (rh Galectin-3 / LGALS3; P17931Met1-Ile250). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | LGALS3 (Galectin 3) is a Protein Coding gene. This gene encodes a member of the galectin family of carbohydrate-binding proteins. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. LGALS3 is a beta-galactoside-binding lectin and plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion, and T-cell regulation. LGALS3 has an important role in tumor progression through inhibition of apoptosis. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Elevated expression of LGALS3 has been demonstrated in the synovium of rheumatoid arthritis (RA). Diseases associated with LGALS3 include Follicular Adenoma and Papillary Carcinoma. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Galectin-3 / LGALS3 protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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