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Anti-Galectin 10 Polyclonal Antibody

Catalog No. TMAB-06285 Copy Product Info
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Anti-Galectin 10 Polyclonal Antibody is a Rabbit antibody targeting Galectin 10. Anti-Galectin 10 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Galectin 10 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-06285

Anti-Galectin 10 Polyclonal Antibody is a Rabbit antibody targeting Galectin 10. Anti-Galectin 10 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Galectin 10 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Galectin 10 Polyclonal Antibody is a Rabbit antibody targeting Galectin 10. Anti-Galectin 10 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat
Verified Activity
1. Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Galectin 10 Polyclonal Antibody, Unconjugated (TMAB-06285) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Galectin 10) Polyclonal Antibody, Unconjugated (TMAB-06285) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Galectin 10) Polyclonal Antibody, Unconjugated (TMAB-06285) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell.
Tissue SpecificityExpressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCharcot-Leyden crystals are endogenous hexagonal bipyramidal crystals present in human tissues and secretions. Presence of Charcot-Leyden crystals correlates with the increased numbers of peripheral blood or tissue eosinophils that occurs with parasitic and allergic processes. Galectin-10, also referred to as Charcot-Leyden crystal (CLC) protein, singularly makes up these crystals. Galectin-10, a member of the galectin family of beta-Galactoside binding proteins that bind to S-type animal lectins, is expressed solely in eosinophilic and basophilic leukocytes. Galectin-10 may possess carbohydrate or IgE-binding activities, and it plays a functional role in the biology of inflammation. Expression of galectin-10 is transcriptionally induced by butyric acid.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Galectin 10
Antigen Species
Human
Gene Name
CLC
Gene ID
Protein Name
Galectin-10
Uniprot ID
Function
May have both lysophospholipase and carbohydrate-binding activities.
Chemical Properties
Molecular WeightTheoretical: 16 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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