Anti-Galectin 1 Polyclonal Antibody is a Rabbit antibody targeting Galectin 1. Anti-Galectin 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Human,Rat,Pig,Cow,Horse,Sheep)

1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Galectin-1 Polyclonal Antibody, Unconjugated (TMAB-06284) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Primary: Anti-Galectin 1 (TMAB-06284) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 14 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Galectin 1

Human

LGALS1

Galectin-1

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.