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Anti-GABRA2 Polyclonal Antibody (Synonyms: GBRA2, gamma-aminobutyric acid receptor subunit alpha-2, gamma amin...)

Catalog No. TMAB-00730 Copy Product Info
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Anti-GABRA2 Polyclonal Antibody is a Rabbit antibody targeting GABRA2. Anti-GABRA2 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

Anti-GABRA2 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-00730
Synonyms GBRA2, gamma-aminobutyric acid receptor subunit alpha-2, gamma aminobutyric acid(GABA) A receptor alpha 2, gamma aminobutyric acid receptor subunit alpha 2, gamma aminobutyric acid receptor alpha 2 subunit, gamma aminobutyric acid A receptor alpha 2, GABRA2, GABRA 2, GABR A2, GABA(A) receptor subunit alpha 2, GABA A receptor subunit alpha 2, GABA A Receptor alpha 2, GABA A Receptor a2, GABA

Anti-GABRA2 Polyclonal Antibody is a Rabbit antibody targeting GABRA2. Anti-GABRA2 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

Anti-GABRA2 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GABRA2 Polyclonal Antibody is a Rabbit antibody targeting GABRA2. Anti-GABRA2 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.
SynonymsGBRA2, gamma-aminobutyric acid receptor subunit alpha-2, gamma aminobutyric acid(GABA) A receptor alpha 2, gamma aminobutyric acid receptor subunit alpha 2, gamma aminobutyric acid receptor alpha 2 subunit, gamma aminobutyric acid A receptor alpha 2, GABRA2, GABRA 2, GABR A2, GABA(A) receptor subunit alpha 2, GABA A receptor subunit alpha 2, GABA A Receptor alpha 2, GABA A Receptor a2, GABA
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated (TMAB-00730) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Cerebrum (mouse) Lysate at 40 μg
Primary: Anti-GABRA2 (TMAB-00730) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa
3. Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-GABRA2 antibody (TMAB-00730), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
4. Sample:
Cerebral cortex (Rat) Lysate at 40 μg
Primary: Anti-GABRA2 (TMAB-00730) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
5. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-GABRA2 (TMAB-00730) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundGABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GABRA2
Antigen Species
Human
Gene Name
GABRA2
Gene ID
Protein Name
gamma-aminobutyric acid receptor subunit alpha-2
Uniprot ID
Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Chemical Properties
Molecular WeightTheoretical: 48 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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