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Anti-GAA Antibody (2K86)

Catalog No. TMAB-06241 Copy Product Info
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Anti-GAA Antibody (2K86) is a Rabbit antibody targeting GAA. Anti-GAA Antibody (2K86) can be used in FCM, WB.

Anti-GAA Antibody (2K86)

Copy Product Info
🥰Excellent
Catalog No. TMAB-06241

Anti-GAA Antibody (2K86) is a Rabbit antibody targeting GAA. Anti-GAA Antibody (2K86) can be used in FCM, WB.

Anti-GAA Antibody
(2K86)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1487-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4707-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GAA Antibody (2K86) is a Rabbit antibody targeting GAA. Anti-GAA Antibody (2K86) can be used in FCM, WB.
Ig Type
IgG
Clone
2K86
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with GAA monoclonal antibody, unconjugated (TMAB-06241) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-GAA antibody (TMAB-06241, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
FCM=1:50-100; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationLysosome. Lysosome membrane.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Concentration1mg/ml
Research BackgroundEssential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans.
Antigen Details
Immunogen
A synthesized peptide: human Lysosomal alpha glucosidase
Antigen Species
Human
Gene Name
GAA
Gene ID
Protein Name
Lysosomal alpha-glucosidase
Uniprot ID
Function
Essential for the degradation of glygogen to glucose in lysosomes.
Subcellular Location : Lysosome. Lysosome membrane.
Chemical Properties
Molecular WeightTheoretical: 105 kDa. Actual: 120, 100, 75 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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