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Anti-GAA Antibody (2K86)
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Catalog No. TMAB-06241
Anti-GAA Antibody (2K86) is a Rabbit antibody targeting GAA. Anti-GAA Antibody (2K86) can be used in FCM, WB.
Anti-GAA Antibody (2K86)
Anti-GAA Antibody (2K86)
Copy Product Info😃Good
Catalog No. TMAB-06241
Anti-GAA Antibody (2K86) is a Rabbit antibody targeting GAA. Anti-GAA Antibody (2K86) can be used in FCM, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-GAA Antibody (2K86) is a Rabbit antibody targeting GAA. Anti-GAA Antibody (2K86) can be used in FCM, WB. |
| Ig Type | IgG |
| Clone | 2K86 |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | 1. 25 ug total protein per lane of various lysates (see on figure) probed with GAA monoclonal antibody, unconjugated (TMAB-06241) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-GAA antibody (TMAB-06241, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | FCM=1:50-100; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lysosome. Lysosome membrane. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Concentration | 1mg/ml |
| Research Background | Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans. |
| Immunogen | A synthesized peptide: human Lysosomal alpha glucosidase |
| Antigen Species | Human |
| Gene Name | GAA |
| Gene ID | |
| Protein Name | Lysosomal alpha-glucosidase |
| Uniprot ID | |
| Function | Essential for the degradation of glygogen to glucose in lysosomes.
Subcellular Location : Lysosome. Lysosome membrane. |
| Molecular Weight | Theoretical: 105 kDa. Actual: 120, 100, 75 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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