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Anti-G6PC Polyclonal Antibody

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Catalog No. TMAB-00724

Anti-G6PC Polyclonal Antibody is a Rabbit antibody targeting G6PC. Anti-G6PC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-G6PC Polyclonal Antibody

Anti-G6PC Polyclonal Antibody

😃Good
Catalog No. TMAB-00724
Anti-G6PC Polyclonal Antibody is a Rabbit antibody targeting G6PC. Anti-G6PC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-G6PC Polyclonal Antibody is a Rabbit antibody targeting G6PC. Anti-G6PC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucose 6 phosphatase alpha) Polyclonal Antibody, Unconjugated (TMAB-00724) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. U-937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TMAB-00724 Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
3. Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human U937cell lysates
Lane 3: Human HL-60 cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human SH-SY5Y cell lysates
Primary: Anti-Glucose 6 phosphatase alpha (TMAB-00724) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 35 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundGlucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Glucose 6 phosphatase alpha
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism of carbohydrates,Other Antibodies,Carbohydrate metabolism,Cancer
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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