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Anti-G-CSF Polyclonal Antibody

😃Good
Catalog No. TMAB-00755
Alias G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33

Anti-G-CSF Polyclonal Antibody is a Rabbit antibody targeting G-CSF. Anti-G-CSF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-G-CSF Polyclonal Antibody

Anti-G-CSF Polyclonal Antibody

😃Good
Catalog No. TMAB-00755Alias G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33
Anti-G-CSF Polyclonal Antibody is a Rabbit antibody targeting G-CSF. Anti-G-CSF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-G-CSF Polyclonal Antibody is a Rabbit antibody targeting G-CSF. Anti-G-CSF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
AliasG-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSF3) Polyclonal Antibody, Unconjugated (TMAB-00755) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSF3) Polyclonal Antibody, Unconjugated (TMAB-00755) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (G-CSF) Polyclonal Antibody, Unconjugated (TMAB-00755) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundGranulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins that regulate haematopoietic cell proliferation and differentiation. G-CSF was isolated initially as a factor supporting the growth of colonies of granulocytes in soft agar cultures. Cells of the monocyte/macrophage lineage are among the most prominent sources of G-CSF, but this factor can also be produced by normal cells of mesodermal origin, including vascular endothelial cells, fibroblasts, and mesothelial cells. Production of G-CSF can be induced in vitro in these cells by a wide variety of stimulatory agents, including LPS, TNF, IL-1, IL-3, I L-4, and IFN-Gamma. G-CSF is likely to play a role in the basal regulation of neutrophil production, and also functions as a primary regulatory factor controlling the neutrophil response to inflammatory stimuli. Furthermore, G-CSF exhibits other biological activities besides the proliferative effects, since G-CSF appears to modulate the distribution of neutrophils and progenitor cells within the body.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CSF3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
CSFs,Angiogenic growth factors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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