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Anti-FUT4 Polyclonal Antibody 2
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Catalog No. TMAB-06216
Anti-FUT4 Polyclonal Antibody 2 is a Rabbit antibody targeting FUT4. Anti-FUT4 Polyclonal Antibody 2 can be used in WB,FCM.
Anti-FUT4 Polyclonal Antibody 2
Anti-FUT4 Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-06216
Anti-FUT4 Polyclonal Antibody 2 is a Rabbit antibody targeting FUT4. Anti-FUT4 Polyclonal Antibody 2 can be used in WB,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-FUT4 Polyclonal Antibody 2 is a Rabbit antibody targeting FUT4. Anti-FUT4 Polyclonal Antibody 2 can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Sample: Large intestine (Mouse) Lysate at 40 μg Primary: Anti-FUT4 (TMAB-06216) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD 2. Sample: LOVo (Human) Cell Lysate at 30 μg Primary: Anti-FUT4 (TMAB-06216) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD 3. Overlay histogram showing HL 60 cells stained with TMAB-06216 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M P1 μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal, Orange line) (1 μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. |
| Tissue Specificity | Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human FUT4 |
| Antigen Species | Human |
| Gene Name | FUT4 |
| Gene ID | |
| Protein Name | alpha-(1, 3)-fucosyltransferase 4 |
| Uniprot ID | |
| Function | May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. |
| Molecular Weight | Theoretical: 58 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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