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Anti-FUT2 Polyclonal Antibody

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Catalog No. TMAB-00721

Anti-FUT2 Polyclonal Antibody is a Rabbit antibody targeting FUT2. Anti-FUT2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-FUT2 Polyclonal Antibody

Anti-FUT2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00721
Anti-FUT2 Polyclonal Antibody is a Rabbit antibody targeting FUT2. Anti-FUT2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-FUT2 Polyclonal Antibody is a Rabbit antibody targeting FUT2. Anti-FUT2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample:
SW480 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-FUT2 (TMAB-00721) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 54 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FUT2) Polyclonal Antibody, Unconjugated (TMAB-00721) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCreates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. FUT2 is expressed on the surface of several human tumor cell lines such as BEL-7404, SPC-A-1, and SGC-7901.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: Human FUT2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Other secreted molecules,Amino acid metabolism,Amino Acids
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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