Anti-FUT2 Polyclonal Antibody is a Rabbit antibody targeting FUT2. Anti-FUT2 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human (predicted:Mouse,Rat,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample:
SW480 (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-FUT2 (TMAB-00721) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 54 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FUT2) Polyclonal Antibody, Unconjugated (TMAB-00721) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: Human FUT2

Human

FUT2

Galactoside alpha-(1, 2)-fucosyltransferase 2

Other secreted molecules,Amino acid metabolism,Amino Acids

Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values.