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Anti-FUT2 Polyclonal Antibody is a Rabbit antibody targeting FUT2. Anti-FUT2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-FUT2 Polyclonal Antibody is a Rabbit antibody targeting FUT2. Anti-FUT2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human (predicted:Mouse,Rat,Pig,Cow,Horse,Rabbit,Sheep) |
Verified Activity | 1. Sample: SW480 (Human) Cell Lysate at 30 μg HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-FUT2 (TMAB-00721) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 54 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (FUT2) Polyclonal Antibody, Unconjugated (TMAB-00721) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. FUT2 is expressed on the surface of several human tumor cell lines such as BEL-7404, SPC-A-1, and SGC-7901. |
Immunogen | KLH conjugated synthetic peptide: Human FUT2 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Other secreted molecules,Amino acid metabolism,Amino Acids |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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