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Anti-FUT11 Polyclonal Antibody

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Catalog No. TMAB-06214

Anti-FUT11 Polyclonal Antibody is a Rabbit antibody targeting FUT11. Anti-FUT11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-FUT11 Polyclonal Antibody

Anti-FUT11 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-06214
Anti-FUT11 Polyclonal Antibody is a Rabbit antibody targeting FUT11. Anti-FUT11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-FUT11 Polyclonal Antibody is a Rabbit antibody targeting FUT11. Anti-FUT11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep)
Verified Activity
1. Sample: Hela Cell (Human) Lysate at 40 μg
Primary: Anti-FUT11 (TMAB-06214) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
2. Sample: HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-FUT11 (TMAB-06214) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FUT11) Polyclonal Antibody, Unconjugated (TMAB-06214) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus > Golgi stack membrane.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundGlycosyltransferases that mediate the regio- and stereoselective transfer of sugars, such as the fucosyltransferases, determine cell surface-carbohydrate profiles, which are essential interfaces for biological recognition processes. Fucosyltransferases (FucTs) catalyze the covalent association of fucose to different positional linkages on sugar acceptor molecules. The carbohydrate moieties that are generated are covalently attached to cell surfaces and are necessary to ensure a surface contour that satisfies a variety of physiological roles. FucT-XI is a 492 amino acid single-pass type II membrane protein that belongs to the glycosyltransferase 10 family. Localizing to Golgi apparatus, FucT-XI may act as a fucosyltransferase and exists as two alternatively spliced isoforms. The gene encoding FucT-XI maps to mouse chromosome 14 A3.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: Human FUT11/FucT-XI
Antigen Species
Human
Gene Name
FUT11
Gene ID
Protein Name
alpha-(1, 3)-fucosyltransferase 11
Uniprot ID
Function
Probable fucosyltransferase.
Chemical Properties
Molecular WeightTheoretical: 56 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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