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Anti-FUS Antibody (3F162)
😃Good
Catalog No. TMAC-01575
Alias Translocated in liposarcoma protein, TLS, RNA-binding protein FUS, POMp75, Oncogene TLS, Oncogene FUS, FUS, 75 kDa DNA-pairing protein
Anti-FUS Antibody (3F162) is a Rabbit antibody targeting FUS. Anti-FUS Antibody (3F162) can be used in FCM,ICC/IF,IHC,WB.
Anti-FUS Antibody (3F162)
Anti-FUS Antibody (3F162)
😃Good
Catalog No. TMAC-01575Alias Translocated in liposarcoma protein, TLS, RNA-binding protein FUS, POMp75, Oncogene TLS, Oncogene FUS, FUS, 75 kDa DNA-pairing protein
Anti-FUS Antibody (3F162) is a Rabbit antibody targeting FUS. Anti-FUS Antibody (3F162) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-FUS Antibody (3F162) is a Rabbit antibody targeting FUS. Anti-FUS Antibody (3F162) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | Translocated in liposarcoma protein, TLS, RNA-binding protein FUS, POMp75, Oncogene TLS, Oncogene FUS, FUS, 75 kDa DNA-pairing protein |
| Ig Type | IgG |
| Clone | 3F162 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of FUS/TLS on K562 cells lysates using anti-FUS/TLS antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FUS/TLS antibody. Counter stained with hematoxylin. 3. ICC staining FUS/TLS in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining FUS/TLS in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining FUS/TLS in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with FUS/TLS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.       |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | EWS and FUS/TLS are nuclear RNA-binding proteins. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1, in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various Ets transcription factors, including Fli-1, ETV1 and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. In human myxoid liposarcomas and myeloid leukemias, chromosomal translocation results in the fusion of the N-terminal region of FUS/TLS with the open reading frame of CHOP. In normal cells, FUS/TLS binds to the DNA-binding domains of nuclear steroid receptors and is also present in subpopulations of TFIID complexes, indicating a potential role for FUS/TLS in the processing of primary transcripts that are generated in response to hormone-induced transcription. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 75 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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