Anti-Furin Antibody (1V361) is a Rabbit antibody targeting Furin. Anti-Furin Antibody (1V361) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1V361

Human,Mouse (predicted:Rat)

1. Western blot analysis of Furin on different lysates with Rabbit anti-Furin antibody (TMAB-06208) at 1/500 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (TMAB-06208, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (TMAB-06208, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (TMAB-06208, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
5. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB: 1:500-200; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Furin

Human

FURIN

Furin

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.