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Anti-FSTL1 Antibody (7R231)
😃Good
Catalog No. TMAY-01332
Alias TSC-36, Fstl, follistatin-like 1, AW107808, AI316791
Anti-FSTL1 Antibody (7R231) is a Rabbit antibody targeting FSTL1. Anti-FSTL1 Antibody (7R231) can be used in FCM,ICC/IF.
Anti-FSTL1 Antibody (7R231)
Anti-FSTL1 Antibody (7R231)
😃Good
Catalog No. TMAY-01332Alias TSC-36, Fstl, follistatin-like 1, AW107808, AI316791
Anti-FSTL1 Antibody (7R231) is a Rabbit antibody targeting FSTL1. Anti-FSTL1 Antibody (7R231) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | TSC-36, Fstl, follistatin-like 1, AW107808, AI316791 |
| Ig Type | Rabbit IgG |
| Clone | 7R231 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of Human FSTL1 expression on HeLa cells . The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human FSTL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Confocal immunofluorescence staining of Human FSTL1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FSTL1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green), countstained with DAPI (blue).Positive staining was localized to cytoplasm. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human FSTL1 (rh FSTL1; TMPY-01106; NP_009016.1; Met1-Ile308). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Follistatin-related protein 1 (FSTL1) is an extracellular glycoprotein whose functional significance in physiological and pathological processes is incompletely understood. Recently, we have shown that FSTL1 acts as a muscle-derived secreted factor that is up-regulated by Akt activation and ischemic stress and that FSTL1 exerts favorable actions on the heart and vasculature. Here, we sought to identify the receptor that mediates the cellular actions of FSTL1. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. FSTL1 is thought to be an autoantigen associated with rheumatoid arthritis. DIP2A functions as a novel receptor that mediates the cardiovascular protective effects of FSTL1. Experiment results have provided in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation, in part, through BMP4 signaling. |
| Conjucates | Unconjugated |
| Others Formats | FITC |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Human FSTL1 protein (TMPY-01106) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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