Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-FSHR Polyclonal Antibody

😃Good
Catalog No. TMAB-00716
Alias LGR1, FSH-R, FSHR, Follitropin receptor, Follicle-stimulating hormone receptor

Anti-FSHR Polyclonal Antibody is a Rabbit antibody targeting FSHR. Anti-FSHR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-FSHR Polyclonal Antibody

Anti-FSHR Polyclonal Antibody

😃Good
Catalog No. TMAB-00716Alias LGR1, FSH-R, FSHR, Follitropin receptor, Follicle-stimulating hormone receptor
Anti-FSHR Polyclonal Antibody is a Rabbit antibody targeting FSHR. Anti-FSHR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5297-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-FSHR Polyclonal Antibody is a Rabbit antibody targeting FSHR. Anti-FSHR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasLGR1, FSH-R, FSHR, Follitropin receptor, Follicle-stimulating hormone receptor
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep,GuineaPig)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FSH receptor) Polyclonal Antibody, Unconjugated (TMAB-00716) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-FSH receptor Polyclonal Antibody, Unconjugated (TMAB-00716) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: HUVEC cells (blue). Primary Antibody: Rabbit Anti-FSH receptor antibody (TMAB-00716), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00716, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
4. Sample:
Testis (Mouse) Lysate at 40 μg
Primary: Anti-FSH receptor (TMAB-00716) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FSHR
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Gonadotrophic axis,More GPCR,GPCR
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-FSHR Polyclonal Antibody | purchase Anti-FSHR Polyclonal Antibody | Anti-FSHR Polyclonal Antibody cost | order Anti-FSHR Polyclonal Antibody