Anti-FRAT2 Polyclonal Antibody is a Rabbit antibody targeting FRAT2. Anti-FRAT2 Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Cow,Sheep)

Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-FRAT2 antibody (TMAB-06160)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human FRAT2

Human

FRAT2

GSK-3-binding protein FRAT2

Positively regulates the Wnt signaling pathway by stabilizing beta-catenin through the association with GSK-3.