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Anti-FPRL1 Polyclonal Antibody 2

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Catalog No. TMAB-00714

Anti-FPRL1 Polyclonal Antibody 2 is a Rabbit antibody targeting FPRL1. Anti-FPRL1 Polyclonal Antibody 2 can be used in WB.

Anti-FPRL1 Polyclonal Antibody 2

Anti-FPRL1 Polyclonal Antibody 2

😃Good
Catalog No. TMAB-00714
Anti-FPRL1 Polyclonal Antibody 2 is a Rabbit antibody targeting FPRL1. Anti-FPRL1 Polyclonal Antibody 2 can be used in WB.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB

Reactivity: Human,Mouse
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Sample:
kidney (Mouse) Lysate at 40 μg
Primary: Anti-FPRL1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 48 kDa
2. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-FPRL1 (TMAB-00714) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 47 kDa
3. Sample:
Lane 1: U937 (Human) Cell Lysate at 30 μg
Lane 2: HL60 (Human) Cell Lysate at 30 μg
Lane 3: Hela (Human) Cell Lysate at 30 μg
Lane 4: HepG2 (Human) Cell Lysate at 30 μg
Lane 5: SW480 (Human) Cell Lysate at 30 μg
Lane 6: LOVO (Human) Cell Lysate at 30 μg
Primary: Anti-FPRL1 (TMAB-00714) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 35 kDa
4. Sample:
Bone (Mouse) Lysate at 40 μg
Primary: Anti-FPRL1 (TMAB-00714) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
5. Sample:
SW480 (Human) Cell Lysate at 30 μg
LOVO (Human) Cell Lysate at 30 μg
Primary: Anti-FPRL1 (TMAB-00714) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundFPRL1 is a low affinity receptor to N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). FPRL1 has been reported in phagocytes, monocytes, neutrophils, differentiated myeloid cells from bone marrow, granulocyte HL-60 cells, and synovial fibroblasts. ESTs have been isolated from blood, leukocyte, lung, and placenta libraries.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FPRL1
Antigen Species
Human
Gene ID
2358
Uniprot ID
Biology Area
Cell adhesion molecules,GPCR
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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