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Anti-FPRL1 Polyclonal Antibody 2 is a Rabbit antibody targeting FPRL1. Anti-FPRL1 Polyclonal Antibody 2 can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human,Mouse |
Ig Type | IgG |
Reactivity | Human,Mouse |
Verified Activity | 1. Sample: kidney (Mouse) Lysate at 40 μg Primary: Anti-FPRL1 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 48 kDa 2. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-FPRL1 (TMAB-00714) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 47 kDa 3. Sample: Lane 1: U937 (Human) Cell Lysate at 30 μg Lane 2: HL60 (Human) Cell Lysate at 30 μg Lane 3: Hela (Human) Cell Lysate at 30 μg Lane 4: HepG2 (Human) Cell Lysate at 30 μg Lane 5: SW480 (Human) Cell Lysate at 30 μg Lane 6: LOVO (Human) Cell Lysate at 30 μg Primary: Anti-FPRL1 (TMAB-00714) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 35 kDa 4. Sample: Bone (Mouse) Lysate at 40 μg Primary: Anti-FPRL1 (TMAB-00714) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa 5. Sample: SW480 (Human) Cell Lysate at 30 μg LOVO (Human) Cell Lysate at 30 μg Primary: Anti-FPRL1 (TMAB-00714) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa |
Application | WB |
Recommended Dose | WB: 1:500-2000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | FPRL1 is a low affinity receptor to N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). FPRL1 has been reported in phagocytes, monocytes, neutrophils, differentiated myeloid cells from bone marrow, granulocyte HL-60 cells, and synovial fibroblasts. ESTs have been isolated from blood, leukocyte, lung, and placenta libraries. |
Immunogen | KLH conjugated synthetic peptide: human FPRL1 |
Antigen Species | Human |
Gene ID | 2358 |
Uniprot ID | |
Biology Area | Cell adhesion molecules,GPCR |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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