Antibody Type: Rabbit Polyclonal

Application: FCM

Reactivity: Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

1. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-FoxP3/PE Conjugated antibody (TMAB-00710-PE)
Dilution: 0.2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature.
2. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (TMAB-00710)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. The MJ (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-FOXP3 antibody (TMAB-00710): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.

FCM

FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human FoxP3

Human

50943

Chromatid Cohesion,FOXP,Regulatory T Cells