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Anti-FOXO4 Antibody (5M471)
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Catalog No. TMAC-01558
Alias trithorax homolog (Drosophila), translocated to 7, RGD1561201, Myeloid/lymphoid or mixed lineage leukemia, MLLT7, Mixed lineage leukemia, MGC120490, MGC117660, FOXO 4, Forkhead box O4, Fork head domain transcription factor AFX1, ALL1-fused gene from X chromosome, Afxh, AFX1, AFX
Anti-FOXO4 Antibody (5M471) is a Rabbit antibody targeting FOXO4. Anti-FOXO4 Antibody (5M471) can be used in WB,ICC/IF,IP,FCM.
Anti-FOXO4 Antibody (5M471)
Anti-FOXO4 Antibody (5M471)
😃Good
Catalog No. TMAC-01558Alias trithorax homolog (Drosophila), translocated to 7, RGD1561201, Myeloid/lymphoid or mixed lineage leukemia, MLLT7, Mixed lineage leukemia, MGC120490, MGC117660, FOXO 4, Forkhead box O4, Fork head domain transcription factor AFX1, ALL1-fused gene from X chromosome, Afxh, AFX1, AFX
Anti-FOXO4 Antibody (5M471) is a Rabbit antibody targeting FOXO4. Anti-FOXO4 Antibody (5M471) can be used in WB,ICC/IF,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-FOXO4 Antibody (5M471) is a Rabbit antibody targeting FOXO4. Anti-FOXO4 Antibody (5M471) can be used in WB,ICC/IF,IP,FCM. |
| Synonyms | trithorax homolog (Drosophila), translocated to 7, RGD1561201, Myeloid/lymphoid or mixed lineage leukemia, MLLT7, Mixed lineage leukemia, MGC120490, MGC117660, FOXO 4, Forkhead box O4, Fork head domain transcription factor AFX1, ALL1-fused gene from X chromosome, Afxh, AFX1, AFX |
| Ig Type | IgG |
| Clone | 5M471 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. ICC staining FOXO4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 2. ICC staining FOXO4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining FOXO4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of 293 cells with FOXO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serine-proline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x;11) translocation of the AFX1 gene, which involves the fusion of a serine-proline-rich sequence of AFX1 to the carboxy terminus of a truncated MLL, results in acute lymphocytic leukemia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 54 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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