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Anti-FOXO3A Polyclonal Antibody

😃Good
Catalog No. TMAB-00709
Alias MGC31925, MGC12739, FOXO3, FOXO2, FOX O3A, Forkhead(Drosophila) homolog(rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead box protein O3A, Forkhead box O3A, FKHRL1P2, FKHRL1, FKHRL 1, FKHR2, DKFZp781A0677, AF6q21

Anti-FOXO3A Polyclonal Antibody is a Rabbit antibody targeting FOXO3A. Anti-FOXO3A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-FOXO3A Polyclonal Antibody

Anti-FOXO3A Polyclonal Antibody

😃Good
Catalog No. TMAB-00709Alias MGC31925, MGC12739, FOXO3, FOXO2, FOX O3A, Forkhead(Drosophila) homolog(rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead box protein O3A, Forkhead box O3A, FKHRL1P2, FKHRL1, FKHRL 1, FKHR2, DKFZp781A0677, AF6q21
Anti-FOXO3A Polyclonal Antibody is a Rabbit antibody targeting FOXO3A. Anti-FOXO3A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
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Product Introduction

Bioactivity
Description
Anti-FOXO3A Polyclonal Antibody is a Rabbit antibody targeting FOXO3A. Anti-FOXO3A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
AliasMGC31925, MGC12739, FOXO3, FOXO2, FOX O3A, Forkhead(Drosophila) homolog(rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead box protein O3A, Forkhead box O3A, FKHRL1P2, FKHRL1, FKHRL 1, FKHR2, DKFZp781A0677, AF6q21
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat rectum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FOXO3A) Polyclonal Antibody, Unconjugated (TMAB-00709) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Uterus tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human DU145 cell lysates
Primary: Anti-FOXO3A (TMAB-00709) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 90 kDa
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Tissue Specificity : Ubiquitous.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FOXO3A
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Forkhead Box,FOXO
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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