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Anti-FOXO3A Polyclonal Antibody is a Rabbit antibody targeting FOXO3A. Anti-FOXO3A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days |
Description | Anti-FOXO3A Polyclonal Antibody is a Rabbit antibody targeting FOXO3A. Anti-FOXO3A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Alias | MGC31925, MGC12739, FOXO3, FOXO2, FOX O3A, Forkhead(Drosophila) homolog(rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead box protein O3A, Forkhead box O3A, FKHRL1P2, FKHRL1, FKHRL 1, FKHR2, DKFZp781A0677, AF6q21 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat rectum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FOXO3A) Polyclonal Antibody, Unconjugated (TMAB-00709) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Uterus tissue lysates Lane 3: Rat Testis tissue lysates Lane 4: Human MCF-7 cell lysates Lane 5: Human DU145 cell lysates Primary: Anti-FOXO3A (TMAB-00709) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 90 kDa |
Application | WB,IHC-P,IHC-Fr,IF,ELISA |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Tissue Specificity : Ubiquitous. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
Immunogen | KLH conjugated synthetic peptide: human FOXO3A |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Forkhead Box,FOXO |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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