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Anti-FOXN1 Polyclonal Antibody

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Catalog No. TMAB-00706
Alias Winged-helix transcription factor nude, Winged helix nude, WHN, Transcription factor winged-helix nude, Rowett nude, RONU, FOXN 1, Forkhead box protein N1, Forkhead box N1, FKHL20

Anti-FOXN1 Polyclonal Antibody is a Rabbit antibody targeting FOXN1. Anti-FOXN1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-FOXN1 Polyclonal Antibody

Anti-FOXN1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00706Alias Winged-helix transcription factor nude, Winged helix nude, WHN, Transcription factor winged-helix nude, Rowett nude, RONU, FOXN 1, Forkhead box protein N1, Forkhead box N1, FKHL20
Anti-FOXN1 Polyclonal Antibody is a Rabbit antibody targeting FOXN1. Anti-FOXN1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-FOXN1 Polyclonal Antibody is a Rabbit antibody targeting FOXN1. Anti-FOXN1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasWinged-helix transcription factor nude, Winged helix nude, WHN, Transcription factor winged-helix nude, Rowett nude, RONU, FOXN 1, Forkhead box protein N1, Forkhead box N1, FKHL20
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-FOXN1 Polyclonal Antibody, Unconjugated (TMAB-00706) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Thymus (Rat) Lysate at 40 μg
Primary: Anti-FOXN1 (TMAB-00706) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FOXN1) Polyclonal Antibody, Unconjugated (TMAB-00706) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-FOXN1 antibody (TMAB-00706)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Blank control (Black line): Hela (Black).
Primary Antibody (green line): Rabbit Anti-FOXN1 antibody (TMAB-00706)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at-20°C. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human FOXN1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
FOXN,Non-CD
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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