Anti-FOXL2 Polyclonal Antibody is a Rabbit antibody targeting FOXL2. Anti-FOXL2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit)

1. Sample:
Lane 1: Human PANC-1 cell lysates
Lane 2: Human K562 cell lysates
Primary: Anti-FOXL2 (TMAB-06129) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45-50 kDa
Observed band size: 55 kDa
2. Sample: A431 Cell (Human) Lysate at 30 μg
Primary: Anti-FOXL2 (TMAB-06129) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45-50 kD
Observed band size: 45 kD
3. Sample: K562 Cell (Human) Lysate at 30 μg
Primary: Anti-FOXL2 (TMAB-06129) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45-50 kD
Observed band size: 50 kD
4. Paraformaldehyde-fixed, paraffin embedded (mouse ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FOXL2) Polyclonal Antibody, Unconjugated (TMAB-06129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human FOXL2

Human

FOXL2

Forkhead box protein L2

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.