Anti-FOXA2 Polyclonal Antibody is a Rabbit antibody targeting FOXA2. Anti-FOXA2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse)

1. Cell: F9
Concentration:1:100
Host/Isotype: Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: TMAB-00703) on F9 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.
2. Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-FOXA2 antibody (TMAB-00703)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Primary: Anti-FOXA2 (TMAB-00703) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
4. 25 μg total protein per Lane of various lysates probed with FOXA2 polyclonal antibody, unconjugated (TMAB-00703) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB,FCM

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human FOXA2

Human

Endoderm,ChIP antibodies,FOXA,Obesity,Endoderm