Anti-Formyl-Histone H2B type 2E (Lys121) Antibody (7L767) is a Rabbit antibody targeting Formyl-Histone H2B type 2E (Lys121). Anti-Formyl-Histone H2B type 2E (Lys121) Antibody (7L767) can be used in FCM, IF, IHC-Fr, IHC-P, WB.

IgG

7L767

Human,Mouse (predicted:Rat)

1. NIH/3T3 (M) cells were treated with or without PMA (100 nM) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Formyl-Histone H2B type 2E (Lys121) monoclonal antibody, unconjugated (TMAB-06109) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Formyl-Histone H2B type 2E (Lys121) antibody (TMAB-06109, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human Histone H2B type 2E around the formylation site of K121

Human

H2BC21

Histone H2B type 2-E