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Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625)
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Catalog No. TMAB-06107
Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) is a Rabbit antibody targeting Formyl-Histone H2B type 2E (Lys109). Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) can be used in FCM, IF, IHC-Fr, IHC-P, WB.
Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625)
Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625)
Copy Product Info😃Good
Catalog No. TMAB-06107
Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) is a Rabbit antibody targeting Formyl-Histone H2B type 2E (Lys109). Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) can be used in FCM, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) is a Rabbit antibody targeting Formyl-Histone H2B type 2E (Lys109). Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) can be used in FCM, IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| Clone | 6G625 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 ug total protein per lane of various lysates (see on figure) probed with Formyl-Histone H2B type 2E (Lys109) monoclonal antibody, unconjugated (TMAB-06107) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Formyl-Histone H2B type 2E (Lys109) antibody (TMAB-06107, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | FCM=1:50-100; IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Immunogen | A synthesized peptide: human Histone H2B type 2E around the formylation site of K109 |
| Antigen Species | Human |
| Gene Name | H2BC21 |
| Gene ID | |
| Protein Name | Histone H2B type 2-E |
| Uniprot ID |
| Molecular Weight | Theoretical: 14 kDa. Actual: 14 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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