Anti-FMNL3 Polyclonal Antibody is a Rabbit antibody targeting FMNL3. Anti-FMNL3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Cow)

1. Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-FMNL3/FITC Conjugated antibody (TMAB-06087-FITC)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (TMAB-06087) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human FMNL3

Human

FMNL3

Formin-like protein 3

FMNL3 is an actin-binding protein that contains a formin homology 2 domain.